MLB Tonight is the signature program that airs on MLB Network and is simulcast on MLB Network Radio. The show offers complete coverage of all Major League Baseball games from 6pm ET till 1am ET during the regular season, and gives news from all 30 MLB teams during the offseason. It is taped live in Studio 3 of the MLB Network facility in Secaucus, New Jersey, but also features segments taped in Studio 42. The program aired from the beginning of Spring Training to the end of the World Series and was replaced in the offseason by Hot Stove, until it started to air in the offseason, and Hot Stove became MLB Network's weekday morning show. The show won the Sports Emmy Award for best Daily Outstanding Studio Show for 2010 and 2011.

MLB Tonight (2009) is currently not available on any services.